HD Integrated Visium Interactive Suite (HiVis)

Project home page is available in github.

Video tutorial is available here.

Installation

To avoid dependency conflicts, we recommend the use of a dedicated conda environment. In a terminal run the command:

conda create -n HiVis python=3.12
conda activate HiVis

We recommend two options to then install HiVis in your virtual environment.

Use the package manager pip to install HiVis. In a terminal run the command:

pip install HiVis

Or clone the project’s Github repository and install it manually with the following commands:

git clone git@github.com:roynov01/HiVis.git
cd HiVis
pip install .

To use Qupath features, such as manual annotations and pixel classifiers, download and install QuPath (>= 0.5.1).

To perform cell segmentation in QuPath, download and install Stardist and/or Cellpose extensions for QuPath.

Tutorials

Please refer to the demo notebooks.

For QuPath, please refer to the tutorial.

Contact

Bug report/feature request via the GitHub issue tracker.

Citation

Novoselsky R*, Golani O*, Barkai T, Kedmi M, Goliand I, Fine M, Kent I, Nachmany I, Itzkovitz S. Subcellular mRNA localization patterns across tissues resolved with spatial transcriptomics. Nature Communications, 2026.

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